Textbook in PDF format

This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 2 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.
Preface
Contributors
Heterogeneous Conjugates
Quantitative Assessment of the Efficacy of Near-Infrared Photoimmunotherapy with Bioluminescence Imaging
Evaluation of the Efficacy of Saracatinib-Loaded Nanoparticles in Lymphatic Metastases of HNSCC with the Aid of Bio...
Reactive Oxygen Species-Responsive and Self-Illuminating Nanoparticles for Inflammation and Tumor Imaging
Antibacterial Activity Evaluation of ZnO, CuO, and TiO2 Nanoparticles in Solution and Thin Films
BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate
Polyhistidine-Tag-Enabled Conjugation of Quantum Dots and Enzymes to DNA Nanostructures
Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes
Protein Fragment-Complementation Assays
Quantitative Imaging of Retinoic Acid Activities in Living Mammalian Cells
Bioluminescence-Based Complementation Assay to Correlate Conformational Changes in Membrane-Bound Complexes with En...
The NanoBiT-Based Homogenous Ligand-Receptor Binding Assay
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-Based Large Tumor Suppressor (LATS) Biosensor
BRET-Based Imaging
Screening of Protein-Protein Interaction Modulators Using BRET-Based Technology
TRUPATH: An Open-Source Biosensor Platform for Interrogating the GPCR Transducerome
MERLIN: A BRET-Based Proximity Biosensor for Studying Mitochondria-ER Contact Sites
Single-Cell NanoBRET Imaging with Green-Range HaloTag Acceptor
Method for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Indicators
BRET Sensors for Imaging Membrane Integrity of Microfluidically Generated Extracellular Vesicles
In Vivo Assessment of Protein-Protein Interactions Using BRET Assay
Live Cell Imaging of ATP Dynamics in Plant Cells
Bioluminescence Resonance Energy Transfer for Global DNA Methylation Quantification
In Vivo Bioluminescent Imaging of Bone Marrow-Derived Mesenchymal Stem Cells in Mice
In Vivo Imaging of Oxidative and Hypoxic Stresses in Mice Model of Amyotrophic Lateral Sclerosis
Instrumentation
ATP Sensing Paper with Smartphone Bioluminescence-Based Detection
Organ Bioluminescence Imaging Under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation
Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo
Bioluminescence-Optogenetics: A Practical Guide
Applications of Bioluminescence-Optogenetics in Rodent Models
One-Channel Microsliding Luminometer for Quantifying Low-Energy Bioluminescent Lights
Compact Eight-Channel Light-Sensing System for Bioassays
Characterization of Firefly Flashes at Various Temperatures in Different Wavelength Regions
Bioluminescent Monitoring of Circadian Rhythms in Isolated Mesophyll Cells of Arabidopsis at Single-Cell Level
Software
Exploring Phylogenetic Relationships and Divergence Times of Bioluminescent Species Using Genomic and Transcriptom...
Index