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Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
Introduction
Physical Fundamentals of Luminescence
Dyes and Fluorochromes
Fluorescent Labels
Spectral Analysis
Fluorescence Instrumental and Techniques
General Fluorescence Reactions
Fluorescent Metal Complexes and Ion Indicators
Luminescent Transition Metal Probes
Lipids
Polysaccharides
Nucleic Acids
Proteins
Enzymes
Vital Probes 1. Cell Organelles
Vital Probes 2. Cell Viability and Signaling
Immunofluorescence and FISH
Luminescent Solid-State Markers
Non-linear Optics
Super-Resolution Microscopy
Subject Index